About 293 cell

ara ara at plaza1.snu.ac.kr
Tue Mar 17 23:09:43 EST 1998


Hi, there!
I have a question about 293 cell (transformed Human Embryonic Kidney
cell).
Human embryonic kidney cells had been transformed by exposing cells to
sheared fragments of adenovirus type 5 DNA. Dr. Graham created this
cell.
However, I don't know exactly which region of adenovirus DNA was
transfected to HEK. Some say "E1A", other say "E1A+E1B". I wonder which
is right.
Do you know?

And another question I have.
Can I buy E1A or E1A+E1B genes? If I can, where do I get them?

Thanks in advance,





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