Mycoplasma infection curing ?

R. Fox richfox at u.washington.edu
Wed Mar 25 23:15:15 EST 1998


I personally like the Gen-Probe in-situ hybridization assay. You can do it
in a few hours and it is realtively tech proof.



On Wed, 25 Mar 1998, Jerome Freed wrote:

> Syzygy wrote:
> 
> > I'm not sure I agree that the infection is coming from an individual.
> > If this were the case I would expect bacterial and yeast infections
> > also to regularly present themselves.
> 
> Often one does not suspect operator error for two reasons:
> 
> 1.  Bacterial and yeast infections lead to turbidity and pH change so
> that the faulty cultures immediately discarded and replaced, or
> 
> 2.  The cultures are routinely carried in the presence of antibiotics
> like penicillin, streptomycin and nystatin, so that errors in technique
> are masked and only the mycoplasmas sneak through. 
> 
> The only way to be sure of what you are doing is to screen for
> mycoplasmas *frequently*.  It is pretty simple to inoculate test
> cultures of 3T3 cells, grow them for a few days, then stain with Hoechst
> 33258 or DAPI and examine with a fluorescent microscope. It is easy to
> recognize the organisms, particularly if you have the courage to keep
> some infested cultures on hand for test purposes. If the courage is
> lacking, some vendors sell fixed and stained positive cells...
> 
> Jerry Freed
> 
> 




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