Vector PCR?

Dr Lester Davids lester at uctgsh1.uct.ac.za
Wed Nov 4 10:41:17 EST 1998


I am looking for some feedback from folks who have successfully PCR'ed
up to 500bp fragments from vector inserts. Are there any "tricks" or
tips needed, compared to PCR from genomic DNA? Also, does it make a
difference if the vector is a protein expression vector as opposed to
normal non expression vectors such as pGEM?

Looking for answers



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