How to prepare a phosphate saline buffer?

M W maaw at
Thu Nov 12 18:18:27 EST 1998

I want a 1/30 M phosphate saline pH 7.4 buffer. I believe this is an
isotonic solution? 

Does it matter if I use Na2HPO4 or NaH2PO4? If I weigh an amount of
phosphate salt and adjust the pH with phosphoric acid (or NaOH),  how much
NaCl should I add to get an isotonic solution?? 


More information about the Cellbiol mailing list