How to prepare a phosphate saline buffer?

M W maaw at freemail.nl
Thu Nov 12 18:18:27 EST 1998


I want a 1/30 M phosphate saline pH 7.4 buffer. I believe this is an
isotonic solution? 

Does it matter if I use Na2HPO4 or NaH2PO4? If I weigh an amount of
phosphate salt and adjust the pH with phosphoric acid (or NaOH),  how much
NaCl should I add to get an isotonic solution?? 

thanks




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