pH varience in RPMI media

darren at indy.bio.uts.edu.au darren at indy.bio.uts.edu.au
Mon Nov 23 19:55:31 EST 1998


We are growing NS-1 cells (for making monoclonal antibodies) in complete
media (10% FCS + commercial liquid RPMI 1640). All seems well, but i've
noticed that the pH of the RPMI seems to shift around even before any cells
are grown in it(usually gets pinker, ie pH increases). Can anyone give me
some guidelines on the "acceptable" range of pH change? What pH parameters do
NS-1 cells take? Should I take this up with our supplier?Thanks

Darren

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