Can Cells Be Released From Colcemid Block?

Sergey Razin iarovaia at ijm.jussieu.fr
Thu Nov 26 07:38:34 EST 1998


In article <73j4f5$1l1$1 at mserv2.dl.ac.uk>, Zhonglin.Chai at med.monash.edu.au
(Zhonglin Chai) wrote:

> Hello Everyone,
> 
>         Do you know if the cells arrested at metaphase by Colcemid (Gibco
> BRL) are able to be released and continue on the cell cycle after washing
> away the reagent?
> 
> 
> 
> 
> ZhongLin Chai, PhD
> ____________________________________________
> Department of Pathology and Immunology
> Monash University Medical School
> Alfred Hospital
> Commercial Rd, Prahran, VIC 3181, AUSTRALIA
> Telephone: (61 3) 9276 2698 (lab)
>            (61 3) 9276 2696 (office)
> Fax:       (61 3) 9276 2731
> email:     zhonglin.chai at med.monash.edu.au
> ____________________________________________

They can be released very easiky, just by washing out colcemid



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