Stable transfection in Jurkats.

delios97 at hotmail.com delios97 at hotmail.com
Wed Nov 25 21:06:55 EST 1998


Use DMRIE-C Reagent, to transfect Jurkat.  Also, don't forget to
activate the cells with PHA and PMA.


On Tue, 24 Nov 1998 11:58:33 +0100, Oskar Fdz-Capetillo
<ggbferuo at lg.ehu.es> wrote:

>    Hi there:
>
>    Has anybody tried to develop stable transformants in Jurkat cells?
>    I am trying to do it but it doesn´t work very well ( with
>lipofectamine transfection ).
>    Any suggestions?
>
>    Oskar





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