Monoclonal vs Polyclonal Ab

Richard P. Grant see_sig at
Mon Nov 30 11:01:43 EST 1998

In article <73ubpj$lo7$1 at>, hoekstra at (Ken
Hoekstra) wrote:

> Are mice then used to produce monoclonal Abs for consistancy, easy of 
> use, etc. reasons? You surely dont get much serum (antibody) from a mouse 
> as compared to a rabbit or cow.

You don't have to.  Once you have a clone producing mAb you can grow as
many cells as you like, and the mAb is in the culture medium. The mAb does
not come from the mouse itself, but from a descendant of one of his spleen
cells.  Hence the difficulty of getting human mAbs . . . ;-)

Pick up a copy of Alberts for an introduction into mAb technology.  


Richard P. Grant MA DPhil     |             rgrant at
Senior R&D Scientist          |             work:
Cambridge Molecular           |      home:           
                    -- Minipreps 'R' Us --

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