Bacterial infection=alkaline phosphatase??
Michael Dorsett Onken
mdonken at artsci.wustl.edu
Thu Oct 8 11:11:50 EST 1998
Henry Wallace (ultrasonic at worldnet.att.net) wrote:
: Hello All:
: I understand that certain bacterial infections
: in humans cause, as an antibody, intestinal
: alkaline phoshatase. I believe one to be the
: bacteria responsible for "sleeping sickness".
: Are there any others?? Are there any www
: sites which I could consult?
: Thank you, Henry Wallace
I may be off base here, but it sounds like you're refering to an ELISA
(enzyme-linked immunosorbent assay). This can be used as a test for the
presence of pathogenic microorganisms in the blood. Essentially, the
blood (or tissue sample) is homogenized and stuck down to the bottom of
several tubes; then antibodies against known pathogens are added to the
tubes, which stick to samples that contain the corresponding microbes. A
second antibody is added which recognizes other antibodies and is
physically linked to Calf Intestinal Alkaline Phosphatase (CIAP), which
causes a color change in the tube when certain chemicals (substrates) are
added. Any sample which contains an infectious agent recognized by the
antibodies will change color, making this a quick, accurate test for known
I ran a MEDLINE search for infection, antibodies, and CIAP, and most of
the records refered to ELISA's.
Mike Onken -. .-. .-. .-. . E-mail:
Molecular Cell ||X|||\ /|||X|||\ /| mdonken at artsci.wustl.edu
Biology Program |/ \|||X|||/ \|||X|| URL:
Washington Univ. ' `-' `-' `-' `- http://madsci.wustl.edu/
More information about the Cellbiol