Separation of HUVEC using a non-enzymatic method?

[Henrik B]

Hi

We intend to do some flow cytometry on HUVEC. The problem is that we want to
keep the surface protein antigens intact. Thus, we don´t want to use
trypsin. Instead we have tried only with EDTA and mechanical processing. It
didn´t work out very well. It this the only way?

Does anyone have a better strategy for this problem?

Best regards

Henrik Blomqvist
Clinical Research Center
Dept. of Internal Medicine
Linköping University Hospital
Sweden