doneill2 at julian.uwo.ca
Thu Oct 15 17:04:00 EST 1998
Due to technical problems (received in sample buffer) I was unable to
quantify the total protein content of my cultured P19 (embryonic stem
cells) cells. I ran equal volumes on a mini-gel and then stained them
with commassie blue. I quantified the entire lane and tried to estimate
relative protein loads. Realizing this is a less desirable method, I
wanted to standardize my protein loads. I was told I could immunoblot
with beta-actin. I'm only concerned about equalling the loads in each
lane rather than the absolute protein content.
Is Beta Actin always proportion to total cellular protein? Do you know
a I might find a basic review on Beta Actin? Often textbooks fail to
mention its relative expression to total protein, or its induction??.
Any thoughts are appreciated.
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