BETA ACTIN?????

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Thu Oct 15 21:34:30 EST 1998


In article <36267150.3A4EDDCD at julian.uwo.ca>, Dave O'Neill
<doneill2 at julian.uwo.ca> wrote:

> HI,
> Due to technical problems (received in sample buffer)  I was unable to
> quantify the total protein content of my cultured P19 (embryonic stem
> cells) cells.  I ran equal volumes on a mini-gel and then stained them
> with commassie blue.

If you have any sample left you could try and quantify the protein
after precipitation with TCA and then a wash (TCA and/or acetone or
methanol) to get rid of interfering substances.  You would only need
eg. 25 ug to do this if you were careful.
     Also, if it was a total protein extract in the sample buffer,
you could possibly assay the DNA content which would at least allow
you to normalise for cell number.
     Good luck,
          Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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