BETA ACTIN?????

Robert Stuart MD drmrmd at mediaone.net
Wed Oct 21 20:12:23 EST 1998


A protein sample in sample buffer is not unquantifiable provided it is
fairly concentrated.  The BCA protein assay (Pierce) is fairly robust to the
presence of SDS, easily up to 0.1%.  The amount of "blue" in all sample
ought to be the same.  Assuming the sample buffer is 2% SDS, and assuming
that the protein concentration is something like 10 ug/50 ul at a minimum
(for pretty gels), even the 20-fold dilution prior to assay would be ~0.01
ug/ul which is do-able with this assay.





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