G418 or neomycin CONFUSION!!

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Wed Oct 28 05:54:32 EST 1998


Hi everyone!
There is some confusion about the antibiotics neomycin and G418 (The
same as geneticin). In the Clontech catalogue you  can read that a
specific vector can be selected with kanamycin in bacteria and with
neomycin in eukaryotic cells as it carries the kan/neo resistance
gene. I therefore ordered Kanamycin and Neomycin as I want to generate
a stable cell line. Now I realize that Neomycin seems to be
ineffective in eukaryotic cells and that G418 has to be used for
eukaryotic cells.
You can imagine that  I am quite angry about those guys from clontech
who put wrong informations into their catalogue. 
So my question is which information is correct: Can neomycin be used
in eukaryotic( mammalian ) cells for selection purposes or not??? And
: Why is there so much confusion about neo- resistance and G418
selection?
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
(remove .nospam before mailing)



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