Transfection of suspension cells ?
martin.Houle at drs.crchul.ulaval.ca
Wed Sep 2 14:43:45 EST 1998
In article <6rdn7p$5ok$1 at green.kreonet.re.kr>, icshin at bioneer.kaist.ac.kr
(Incheol Shin) wrote:
As far as HL-60 cells are concerned, the only approaches that work for
transfection are electroporation and virus-mediated transfection.
I have tried lipofectamine, superfect, effectene, calcium phosphate and
others I know have tried the dextran method without success.
Furthermore, retroviral transfection of HL-60 cells give quite poor
results: about 1-2% transfection efficency. Electroporation remains the
best alternative for transfection HL-60 cells. Also, I recommend you use a
lot of DNA in your transfections. I used 40micrograms per transfection.
Hope this helps
Martin Houle M.Sc.
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