I'm student and I'm currently working with a multichain protein (3 chains).
In a first time, I would like to obtain stable clone with 2 chains of this
protein stbly integrated but I have some problems. Indeed, I obtained only
stable clone for A or B, but no AB. Each chain is in SV40 vector (NeoR)
and I made a cotransfection of A and B containing vectors. I use phosphate
calcium transfection. Has any body an idee to obtain an AB stable clone ?
Is it possible to make multiselection with mammamlian cell (Zeo + Neo + Puro
+...) without kill cells ?
Thanks in advance
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