I would like to obtain a stable clone for 2 chains of a protein, but I have
Bernard Murray suggest me, with other possibilities, to use bicistronic vector.
Do you think that it is possible to obtain A and B chains with the following
vector (if it is possible for me to construct it !) :
....---pCMV--A chain--IVS--IRES--B chain--pA---....---pSV40--NeoR--pA--...
Indeed, this construction (in pCDNA3) results of the insertion of a big
insert into the MCS of this vector. Do you think that this construction
could be functionnal ? I'm not expert in this problem, but I wonder if it
is possible to construct this vector because the bicistronic vectors
generally encode for one protein and a resistance, and not two proteins and
Thank you in advance for your help.
P.S. : Thank you very much Bernard for your very interesting answer !!
Faculte de Medecine de Rouen
22 Boulevard Gambetta
76183 ROUEN Cedex
Tel : (33)-02-35-14-82-80
Fax : (33)-02-3514-82-37
E-Mail : INSERM-U295 at univ-rouen.fr