Thymus macrophage isolation

Christopher Hatton (remove_this) "CDH8" at le.ac.uk
Tue Sep 22 07:40:30 EST 1998


Christopher Hatton wrote:
> 
> Hi Youngkyun Lee,


I've just found this paper aswell, but for mouse, they do 2 enzymic
digests of whole thymus.

D. DeLuca et al(1995) Programmed differentiation of murine
thymocytes...etc.Journal of Immunological Methods 178 p13-29

Collagenase for 30 mins followed by 0.12-0.25% trypsin.

again should be useful but will still need to purify the thymic nurse
cells by antibodies.

Christopher Hatton
CMHT
Leicester
(UK)



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