THP-1 2D-PAGE

Ralf Breuker Ralf.Breuker at ruhr-uni-bochum.de
Wed Sep 23 01:04:35 EST 1998


Dear Dirk,

>In an extract of THP-1 cells there are two very abundant proteins, one
>of Mr 43000 and one of Mr 55000. They both have a pI between 4 and 6.
>I believe the p43 could be beta actin and the p55 tubulin.


I did never make a 2D-PAGE of these cells, but your suggestion is very
probable, since actin and tubulin belong to the most abundant proteins in
cells.

Greetings from the tubulin labs...

Ralf

Dr. Ralf Breuker (Diplombiologe)
Lehrstuhl für Zellmorphologie, NDEF 05, Ruhr-Universität Bochum, 44780
Bochum
Tel.: (49) 0234 7005812; FAX: (49) 0234 7094313
Stockumerstr. 218, 58454 Witten
Tel.: (49) 02302 47823; FAX: (49) 089-66617-32787
http://homepage.ruhr-uni-bochum.de/Ralf.Breuker/





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