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Chicken red blood cells

dmorgan dormor3 at concentric.net
Sat Sep 26 20:45:45 EST 1998


Just curious..since chicken RBCs are nucleated, do they pellet in LSM or stay in
the interface as human nucleated RBCs tend to do.

Olivier Gandrillon wrote:

> Dear HSJ
>
> We usually eliminate *most* of the RBC by passsing  the bone marrow
> cells on top of a gradient of Lymphocyte Separation Medium (or any
> approachiing type of viscous solution). Please do not hesitate if you
> need more infos.
>
> Hope this helps.
>
> Olivier
>
> HSJ wrote:
> >
> > Dear Sirs,
> > I want to eliminate the red blood cells from PBMCs of chicken. I've alredy
> > searched in internet and I didn't find nothing. If one of you could help
> > me....
> > Thanks in advance,
> >                           Hamilton.




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