They DO pellet. I guess what's important here is cellular density, that
is only marginally affected by nucleus. Human nucleated cells are less
differentiated that is less dense (strong reduction in cell size is seen
both in mammalls and chicken RBC), which could explain why they do not
>> Just curious..since chicken RBCs are nucleated, do they pellet in LSM or stay in
> the interface as human nucleated RBCs tend to do.
>> Olivier Gandrillon wrote:
>> > Dear HSJ
> > We usually eliminate *most* of the RBC by passsing the bone marrow
> > cells on top of a gradient of Lymphocyte Separation Medium (or any
> > approachiing type of viscous solution). Please do not hesitate if you
> > need more infos.
> > Hope this helps.
> > Olivier
> > HSJ wrote:
> > >
> > > Dear Sirs,
> > > I want to eliminate the red blood cells from PBMCs of chicken. I've alredy
> > > searched in internet and I didn't find nothing. If one of you could help
> > > me....
> > > Thanks in advance,
> > > Hamilton.