In article <6uotg5$vhi at f1n1.spenet.wfu.edu>,
kuhnray at wfu.edu (Raymond E. Kuhn, Ph.D.) wrote:
> We are wanting to do some assays on chemotaxis of fibroblasts. We are
> new to this aspect of research and are fairly clueless, even after
> looking through the literature, on where to buy the chambers, how to
> set them up and how to run the assays. The literature generally just
> states that "using Boyden chambers."
> If anyone could provide me with a source of the chambers, a step-wise
> approach to using them and any other simplified information, I would
> really appreciate it.
Ah yes, the Boyden chamber....brings back memories of college. This is
a classic folks. In the old days...ahem...if you needed something
weird, you needed Bellco. Sure enough, a quick check reveals catalog
#1943-Chemotaxis Chamber and the following references: Baum, Mowat, and
Kirk, 1971. Amer. Journal. Lab. Clin. Med 77(3):501-509. and, as one
might have expected, Boyden 1962. Journal Expt. Med 115:453.
Basically we are talking two chambers separated by a filter that cells
can squeeze through. Why did the cell cross the filter? Why, to get to
the other side of course...where, presumably, an effective
Bellco can be reached at 1-800-257-7043.
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