Most likely you have also noticed a more rapid evaporation in the outer wells
also. There really is not much you can do to ensure all wells support growth at
same rate. I would suggest, though, using only the inside wells and filling the
outside rows with sterile water to minimize evaporation. May sound somewhat
wasteful of these wells, but there it is.
S. McKay wrote:
> Hi! I've been performing cell proliferation assays on JEG cells in 96-well
> plates. Have noticed that the rate of cell growth in the outer rows of
> cells is considerable less that their replicas further towards the centre
> of the plate. Was wondering if anyone else had observed this problem and
> how they resolve it.
>> Sarah-Jane McKay