Neal Melvin <nrmelvin at acs.ucalgary.ca> wrote: >How does sucrose cryoprotect a tissue sample??? In other words, what >does the sucrose due to the tissue to make it less vulnerable to >freezing artifacts? It dehydrates the cell and reduces the extracellular salt concentration colligatively during freezing. Ken Muldrew kmuldrew at acs.ucalgary.ca