voltage clamping cardiac cells

Hasan Almulla halmulla at CONNECT4FREE.NET
Thu Jul 22 16:31:25 EST 1999 

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Hi everybody

Currently I'm studying magnesium regulation in cardiac cells using both =
fluorescent dye furaptra and voltage clamping combined.I have been =
trying votage clamp rat ventricular myocytes using patch electrodes (2-5 =
Mohm) but the rate of success is very low indeed, they just won't seal. =
any tips?=20

Thanks for your help
Hasan Almulla

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<META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR>
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<DIV><FONT color=3D#000000 size=3D2>Hi everybody</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 size=3D2>Currently I'm studying magnesium =
regulation in=20
cardiac cells using both fluorescent dye furaptra and voltage clamping=20
combined.I have been trying votage clamp rat ventricular myocytes using =
patch=20
electrodes (2-5 Mohm) but the rate of success is very low indeed, they =
just=20
won't seal. any tips? </FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 size=3D2>Thanks for your help</FONT></DIV>
<DIV><FONT color=3D#000000 size=3D2>Hasan =
Almulla</FONT></DIV></BODY></HTML>

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