Permount going cloudy

James King jking001 at nimbus.ocis.temple.edu
Fri Mar 5 13:28:26 EST 1999


On 5 Mar 1999, Peter Ashby wrote:

> In article <7bnele$in3$1 at nntp.ucs.ubc.ca>, hoekstra at unixg.ubc.ca (Ken
> Hoekstra) wrote:
> 
> > I've recently mounted some slides with Permount (Fisher Science). 
> > However, after a few days, the area around my tissue under the coverslip 
> > went cloudy, making the tissue very difficult to see. Tissue was 
> > dehydrated through EtOH series and xylene and then mounted. The Permount 
> > is not yet expired but for some reason changes from clear to cloudy in 
> > few days. No air bubbles under coverslip. 
> > 
> > Any suggestions? Can you remount slides by taking them back to xylene?
> 
> You can remount by taking back to xylene, no problem. It occurs to me that
> if your sections aren't fully dehydrated or cleared of ethanol it could
> give the symptom you describe. Try replacing all your final dehydration
> solutions.
> 
> Peter
> 
	I believe Peter is correct.  It sounds like those sections are not
fully dehydrated.  Definitely give it another go by taking the coverslips
off with xylenes and dehydrating with fresh solution.

Good luck,
Jim





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