Permount going cloudy
jking001 at nimbus.ocis.temple.edu
Fri Mar 5 13:28:26 EST 1999
On 5 Mar 1999, Peter Ashby wrote:
> In article <7bnele$in3$1 at nntp.ucs.ubc.ca>, hoekstra at unixg.ubc.ca (Ken
> Hoekstra) wrote:
> > I've recently mounted some slides with Permount (Fisher Science).
> > However, after a few days, the area around my tissue under the coverslip
> > went cloudy, making the tissue very difficult to see. Tissue was
> > dehydrated through EtOH series and xylene and then mounted. The Permount
> > is not yet expired but for some reason changes from clear to cloudy in
> > few days. No air bubbles under coverslip.
> > Any suggestions? Can you remount slides by taking them back to xylene?
> You can remount by taking back to xylene, no problem. It occurs to me that
> if your sections aren't fully dehydrated or cleared of ethanol it could
> give the symptom you describe. Try replacing all your final dehydration
I believe Peter is correct. It sounds like those sections are not
fully dehydrated. Definitely give it another go by taking the coverslips
off with xylenes and dehydrating with fresh solution.
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