UV sterilization???

Biology mpiraee at hotmail.com
Wed Mar 10 19:01:28 EST 1999

UV will work only on surface and for asir sterilization, Sometimes for water in
thin tubes (while flowing).
Why not using autoclave for equipments you listed??

Jens Hansson wrote:

> Hi!
> I have an UVP CL-1000 crosslinker (wave length 254 nm), which I have used to
> UV crosslink DNA to nylon membranes. However, I also like to be able to
> sterilize lab.equipment like plastic tubes, scissors etc, but I do not know
> how much 254 nm irradiation is needed. I guess it depends on the material,
> the distance from the light-bulbs etc. However, the UVP CL-1000 crosslinker
> is a box, with the light-bulbs in the roof, and the item is placed on the
> floor, about 18 cm between the floor and roof. The preprogrammed UV exposure
> setting is 120 000 microjoules per cm2, the recommended exposure for DNA
> crosslinking to membranes, but I guess sterilization requires more
> irradiation. Any suggestions??
> /Jens Hansson

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