Help!!!!

[Uma chrissie]

"Cindy Tong" <93063454 at one2free.com.hk> wrote:
>I got a problem recently. I have to count the mitotic index on the HeLa>cell. I try to use propidium iodide to stain my HeLa cell. Then I use the
>fluorescent microscope to find out the cell in M phase. But the problem is
>that it is very  difficult to distingush which cell is in M phase. Dose
>anyone who have the experience to count the mitotic index before? If so,
>please tell me what kind of method can I fix the cell on the slide? So what
>kind of staining dye is the best to stain the cell?
>
Dear Cindy Tong,

The most common fixative uesd is Carnoy's fixative which is basically 3parts methanol and 1 part acetic acid.
Your cells have to be thoroughly washed in fixative before proceeding to staining.
If you are only required to do a chromosome count without any banding then Giemsa stain is sufficient.

Yours truly,
Chrissie
vum at np.edu.sg
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