PCR cloning

Edgar Vogt ejvogt at yahoo.com
Thu Nov 18 17:44:09 EST 1999


Tim Poulsen <tim.poulsen at get2net.dk> schrieb in im Newsbeitrag:
7YMI3.1403$AX3.529 at news.get2net.dk...
>
> Entomology wrote in message
> <3.0.3.32.19990923164031.0069c500 at 192.168.0.1>...
> >Hi,  I'm trying to cloning a PCR product by
> >
> >blunt-end ligation. Is there any suggestion for efficient
> >
> >cloning? Can I treat PCR product before ligation using klenow
polymerase.I
> have to clone my PCR products using smaI blunt
> >
> >site,
> >
> >Milind Ratnaparkhe
> >
> ><color><param>0000,0000,ffff</param>Persons Outside DRDE please note :
> >
> >
> >
> >REPLY AT : drde at gwr1.dot.net.in
> >
>
>
> Yoy can bye a cloning kit at In Vitrogen, that allows you to clone a blunt
> end PCR product with 95-100% effect.
>
> Tim Poulsen
>
>






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