Primary cortical cultures

Mano mdep at
Tue Oct 5 21:11:57 EST 1999

hi all,
    I am preparing primary cultures from E16-E18 mouse embryos.  I would
like to get single neuronal cells, however I end up with large
aggregates with large neuritic outgrowths. This makes it difficult for
single cell analysis.  Any suggestions? Your attention to this matter
would be appreciated greatly.


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