Primary cortical cultures

Sean Patterson seanpat at FMED2.UNCU.EDU.AR
Fri Oct 8 08:02:29 EST 1999


>hi all,
>    I am preparing primary cultures from E16-E18 mouse embryos.  I would
>like to get single neuronal cells, however I end up with large
>aggregates with large neuritic outgrowths. This makes it difficult for
>single cell analysis.  Any suggestions? Your attention to this matter
>would be appreciated greatly.
>
>Thanks

How are you disaggregating your cells? You may need to increase your enzyme
treatment or trituration procedures.

Sean


Sean Patterson, Ph.D.
Catedra de Fisiologia, CC33
Facultad de Ciencias Medicas
Universidad Nacional de Cuyo
Mendoza, Argentina
Tel: (0261) 430-9385
Fax: (0261) 449-4117
e-mail: seanpat at fmed2.uncu.edu.ar





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