coimmunostaining of O1/O4 with MAG antibody

[Stephane Genoud]

Hi,

Does anybody know how I could do a coimmunostaining on cryosections with 2
antibodies which don't have the same conditions: that is, we don't have to
use any  detergent for the O1/O4 antibody but we have to for the MAG
antibody. The idea would be  to do first the O1/O4 staining, fix then the
reaction with something strong ( WHAT FOR  EXAMPLE?) and then continue with
the MAG staining.
So, has someone already try to do that and if yes what strong fixative
could we use to fix  the O1/O4 reaction before continuing with the MAG
reaction?

Any suggestions will be very appreciated,
Thanks



Stephane Genoud
Institute of Cell Biology
Swiss Federal Institute of Technology
ETH-Hoenggerberg HPM E23
CH-8093 Zürich, Switzerland

Phone:	(+41) 1 633  33 48
FAX :	(+41) 1 633  10 69