background from Streptavidin-FITC

Jie Zhou zhou at MPASMB.DESY.DE
Mon Oct 18 07:41:22 EST 1999


Hi,

I use streptavidin-FITC to do immunofluresence, the primary antibody was
biotinylated.
But I got a very high bacckground, the whole cells were stained.

Who has experiences about this, please help me.

Thanks.

Jie Zhou





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