Why luciferase but not beta-gal or CAT?

Annette C. Hollmann ah690549 at bcm.tmc.edu
Wed Oct 20 07:42:14 EST 1999


In article <199910201214.FAA28419 at net.bio.net> tony_shen at 263.net writes:
>I wonder why researchers use luciferase but not beta-gal or CAT
>for reporter assay nowadays.
>Is it because it is more sensitive or accurate?
>

It is more sensitive, it has a wider linear range, and it's faster to do.

Annette


























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