Why luciferase but not beta-gal or CAT?

beckwith at netcomuk.co.uk beckwith at netcomuk.co.uk
Wed Oct 20 12:48:57 EST 1999


Annette C. Hollmann wrote:
> 
> In article <199910201214.FAA28419 at net.bio.net> tony_shen at 263.net writes:
> >I wonder why researchers use luciferase but not beta-gal or CAT
> >for reporter assay nowadays.
> >Is it because it is more sensitive or accurate?
> >
> 
> It is more sensitive, it has a wider linear range, and it's faster to do.
> 
> Annette



What about a 20min CAT assay?? Lose slightly on sensitivity, but it is
rapid.

Jason



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