Can Beta-Galactosidase be used as a reporter gene?

Annette C. Hollmann ah690549 at bcm.tmc.edu
Fri Oct 22 07:56:32 EST 1999


In article <199910221027.DAA01262 at net.bio.net> tony_shen at 263.net writes:
>Nowadays most scientist use lusiferase or CAT as a reporter gene.
>But they need a luminometer or scintillation counter to detect the product. 
>Can Beta-Galactosidase be used as a reporter gene?
>If so, I could do the reporter assay with a microplate reader.
>
>I want to know whether it is practicable. 

If you've got a reasonable transfection efficiency, it works quite well.
You can use the ONPG substrate and then measure the absorbance in either a
plate reader or a spectrophotometer.
There are also newer, more sensitive substrates available for colorimetric
detection.

Annette




























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