help
alicemx at my-deja.com
alicemx at my-deja.com
Thu Oct 28 10:45:56 EST 1999
I got a sequence from genbank, from the introduction you can see that
the exon start from 62, but if you try to translate the sequence to
amino acid sequence you will find you meet two stop codons, gaggatctga
gaaatgtgac , how comes? it is not reasonable? anyone can give me an
explaination? thank you very much!
LOCUS MUSTCBA01 510 bp DNA ROD 27-APR-
1993
DEFINITION Mouse T-cell receptor germline beta-chain gene constant
region
(CT), exon 1.ACCESSION M26053 M23826NID g201235
VERSION M26053.1 GI:201235KEYWORDS T-cell; T-cell receptor.
SEGMENT 1 of 4SOURCE Mouse (cell line B10.A) liver DNA.
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata;
Mammalia;
Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE 1 (bases 1 to 510)
AUTHORS Gascoigne,N.R.J., Chien,Y.-h., Becker,D.M., Kavaler,J. and
Davis,M.M.
TITLE Genomic organization and sequence of T-cell receptor beta-
chain
constant- and joining-region genes
JOURNAL Nature 310, 387-391 (1984) MEDLINE 84270704
FEATURES Location/Qualifiers source 1..510
/organism="Mus musculus"
/db_xref="taxon:10090" intron <1..61
/gene="TCR" exon 62..>436
/gene="TCR" /number=1
CDS join(62..436,M26054.1:61..78,M26055.1:29..135,
M26056.1:82..102) /partial
/gene="TCR" /codon_start=3
/product="T cell receptor"
/protein_id="AAA40199.1"
/db_xref="PID:g201240"
/db_xref="GI:201240"
/translation="DLRNVTPPKVSLFEPSKAEIANKQKATLVCLARGFFPDHVELSW
WVNGKEVHSGVSTDPQAYKESNYSYCLSSRLRVSATFWHNPRNHFRCQVQFHGLSEED
KWPEGSPKPVTQNISAEAWGRADCGITSASYQQGVLSATILYEILLGKATLYAVLVST
LVVMAMVKRKNS" intron 437..>510
/gene="TCR"BASE COUNT 137 a 131 c 141
g 101 t
ORIGIN Chromosome 6.
1 ttacaagatc aaggcagatc cagatagctc tcagaccatt cgtactctct
ttactttcca
61 gaggatctga gaaatgtgac tccacccaag gtctccttgt ttgagccatc
aaaagcagag
121 attgcaaaca aacaaaaggc taccctcgtg tgcttggcca ggggcttctt
ccctgaccac
181 gtggagctga gctggtgggt gaatggcaag gaggtccaca gtggggtcag
cacggaccct
241 caggcctaca aggagagcaa ttatagctac tgcctgagca gccgcctgag
ggtctctgct
301 accttctggc acaatcctcg caaccacttc cgctgccaag tgcagttcca
tgggctttca
361 gaggaggaca agtggccaga gggctcaccc aaacctgtca cacagaacat
cagtgcagag
421 gcctggggcc gagcaggtaa gtgggacttg gggagctggc aagaagaata
agcatcagtc
481 gtgccaatga aaaaatggaa agatgcaggt
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