AFS7 at le.ac.uk
Thu Sep 9 17:00:44 EST 1999
Ken Hoekstra wrote:
> Does anyone have any ideas (procedures, etc.) for bilirubin analysis from
> tissue homogenates?
I've never done it myself, but a quick PubMed search revealed the
1. Artif Cells Blood Substit Immobil Biotechnol 1999 Jul;27(4):357-65
Comparision of bilirubin conjugation in encapsulated hepatocytes,
hepatocyte homogenate and intact hepatocytes. Bruni S, Chang TM
2. Hepatology 1981 Nov-Dec;1(6):622-7
Bilirubin mono- and diglucuronide formation by human liver in vitro:
assay by high-pressure liquid chromatography. Chowdhury JR, Chowdhury
NR, Wu G, Shouval R, Arias IM
They both used HPLC.
The following is too old to have a PubMed abstract, but sounds
3. Anal Biochem 1972 Dec;50(2):652-5
Determination of bilirubin in liver homogenate with diazotized
p-iodoaniline. Improvement of color recovery by incorporation of
antioxidant (2,6-di-tert-butyl-p-cresol) in the reaction system.
Mertens BB, Van de Vijver M, Heirwegh KP
> Thanks for your help.
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