Mutagenesis Problem?

A.F. Simpson AFS7 at le.ac.uk
Fri Sep 10 17:12:32 EST 1999


Dr Lester Davids wrote:
> 
> Hi All,
> 
> However, when we try to express these mutants
> in JM109's and try to purify them (our gene is in a HisTag vector for
> purification), we get no purified protein and no activity. Recently
> we've created a "safe-mutant" (ie. same aa, different last nucleotide
> eg.instead of TTA we made leucine TTG) and we still have the same no-go
> result.

Does the wild-type protein express alright or not?

> Lester Davids

love
Anna



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