quantification of protein
AFS7 at le.ac.uk
Wed Sep 22 18:32:23 EST 1999
Richard P. Grant wrote:
> In article <37E957AE.1F55 at le.ac.uk>, "A.F. Simpson" <AFS7 at le.ac.uk> wrote:
> >There's always the risk that your housekeeping protein could also be
> >affected by the antibiotic.
> well, yes, but then you wouldn't see a relative difference and so the
> effect is non-specific.
Only if the effect of the antibiotic is to increase or decrease the
expression of both housekeeping and target protein by the same amount.
If it increases one and decreases the other, or specifically alters the
expression of the housekeeping gene, it would make the total protein
loading look unequal when was not.
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