PCR cloning

Entomology ento at GWR1.DOT.NET.IN
Thu Sep 23 06:06:14 EST 1999


Hi,  I'm trying to cloning a PCR product by

blunt-end ligation. Is there any suggestion for efficient

cloning? Can I treat PCR product before ligation using klenow polymerase.I have to clone my PCR products using smaI blunt

site,  

Milind Ratnaparkhe

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