Hi, I'm trying to cloning a PCR product by blunt-end ligation. Is there any suggestion for efficient cloning? Can I treat PCR product before ligation using klenow polymerase.I have to clone my PCR products using smaI blunt site, Milind Ratnaparkhe <color><param>0000,0000,ffff</param>Persons Outside DRDE please note : REPLY AT : drde at gwr1.dot.net.in </color>