PCR cloning

Tim Poulsen tim.poulsen at get2net.dk
Thu Sep 30 12:26:41 EST 1999


Entomology wrote in message
<3.0.3.32.19990923164031.0069c500 at 192.168.0.1>...
>Hi,  I'm trying to cloning a PCR product by
>
>blunt-end ligation. Is there any suggestion for efficient
>
>cloning? Can I treat PCR product before ligation using klenow polymerase.I
have to clone my PCR products using smaI blunt
>
>site,
>
>Milind Ratnaparkhe
>
><color><param>0000,0000,ffff</param>Persons Outside DRDE please note :
>
>
>
>REPLY AT : drde at gwr1.dot.net.in
>


Yoy can bye a cloning kit at In Vitrogen, that allows you to clone a blunt
end PCR product with 95-100% effect.

Tim Poulsen





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