BMBPMN at leeds.ac.uk
Mon Apr 3 08:37:28 EST 2000
I have an old question, but one which no-one has ever satisfactorily answered.
What is Passage number?
or perhaps more specifically
Does passage number actually mean anything?
I am well aware of what a passage is, but i've never understood why it is so
significant: If your cells are confluent (and thus ready to passage) at 16000
cell per cm sq, and you seed Flask A at 8000 cells per cm sq and Flask B at
1000 cells per cm sq, then by the time you passage them the cells in Flask B
will have gone thru 4 population doublings and those in Flask A only 1,
despite them both advancing only one passage number.
Surely population doubling is more important, and passage number
irrelevant without density info?
I appreciate that i probably need to get out a bit more.
bmbpmn at leeds.ac.uk
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