Help! neuronal cell culture problem
rcb5 at msn.com
Wed Aug 9 18:25:52 EST 2000
While there was no hint in your discussion, I believe your cells were
exposed to fluctuating electromagnetic fields that is a harmonic of 10
hertz. Perhaps, a florescent light, a fan near by, a cellar phone, a
person near by with a satellite dish.
----- Original Message -----
From: "Don Cates" <cates at cc.umanitoba.ca>
To: <cellbiol at hgmp.mrc.ac.uk>; <neur-sci at hgmp.mrc.ac.uk>
Sent: Wednesday, August 02, 2000 9:21 AM
Subject: Help! neuronal cell culture problem
> I've been doing cell culture of dissociated neurons from fetal rat
> medulla for several years. Recently the wheels have fallen off.
> I use 35 mm plastic dishes with a collagen (Vitrogen) coating. I then
> plate with neuron free medullary background cells for a week or more,
> then plate with my dissociated cells (mince with scissors, treat with
> trypsin for 15-20 min, gently triturate). The last few times I have done
> this, the cells seem to lift off most of the dish and form long, thick
> 'ropes' of cells which include the collagen and background cells. I've
> replaced my various media and components with fresh material with no
> luck. It is not invariable, I get the odd good dish.
> Can anyone suggest anything?
> Don Cates
> cates at cc.umanitoba.ca
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