Methotrexate amplification

Student mucineer at iname.com
Tue Aug 15 06:36:42 EST 2000


Dear All

I have a question which is puzzling me. After transfection and selection
(with media lacking ribo/deoxyribonucleosides and G418) of a CHO cell line
trasnfected with my plasmid of interest, it is possible to "amplify" the
copy number of the gene in the host chromosome by adding increasing amounts
of methotrexate.

Exactly how do these pieces of DNA which is already incorporated into the
host chromosome "increase in number"?

Any insights will be appreciated. Thanks.











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