Suitable fibroblast for Horse Serum

Paul pd1 at mole.bio.cam.ac.uk
Mon Aug 21 05:31:31 EST 2000



"Rone-Clarke, Barbara" wrote:

> I am trying to find a suitable fibroblast cell line, preferably lung
> derived, which will grow well in media supplemented with horse serum.
>
> I have tried MRC-5 and WI38 cells in RPMI with mixed success so far.
>
> Any help would be appreciated
>
>

A colleague successfully grew COS cells in horse serum (to generate Q
deficient tRNAs).  He said they looked a bit granular after a while but
the division time didn't change noticeably.

Paul Digard







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