transfection

christian christian.troidlNOchSPAM at kerckhoff.mpg.de.invalid
Fri Feb 4 09:38:30 EST 2000




In article <067edcc0.ed3f035c at usw-ex0101-004.remarq.com>,
yinxin <yinxinNOyiSPAM at hotmail.com.invalid> wrote:
> Hi all there,
> i like to know if anybody has any good protocol for
> transfection to
> primary cell, such as myoblast and cardiacmyocyte?
> thanks.
> yinxin
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dear yinxin

maby you should try out the "Gen-Gun" provided by BioRad.
we are also trying out this new method on cardiocytes. but
so
far we haven`t a stable protocol for this system. but some
other groups have good results with this system.
for myoblasts it is maby possible to use the FuGENE 6 Kit
from Roche

greatings christian




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