christian.troidlNOchSPAM at kerckhoff.mpg.de.invalid
Mon Feb 7 05:04:45 EST 2000
In article <spam-0402001429280001 at macmac-2.ucsf.edu>,
spam at 127.0.0.1 (Bernard Murray, PhD) wrote:
> In article
> <019bf6ce.36646537 at usw-ex0109-069.remarq.com>,
> <christian.troidlNOchSPAM at kerckhoff.mpg.de.invalid>
> > I want to count beta-Gal transfected cardiocytes on a
> > petridish!
> Rather than a gridded dish you can use a microscope
> insert (graticule) that has a grid upon it. The other
> way is with a graticule that has a random pattern of
> on it and you then count the proportion of dots
> landing on
> stained cells to give yourself a transfection
> I've used the former method for a variety of b-gal
> cell lines.
> Bernard P. Murray, PhD
> bpmurray at cgl . ucsf . edu
> Department of Cellular & Molecular Pharmacology, UCSF
thank you very much for your answer to my request.
as you maby realized I'm german and I have problems with the
word "graticule". could you specifie what you mean by that.
Max-Planck-Institut for Physiol. and Clinical Science
Dept. of Exp. Cardiology
Bad Nauheim, Germany
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