3' end of RNA

ephray ephray at home.com
Sun Feb 20 15:46:24 EST 2000


Does anybody know a way to design a vector (plasmid or virus) which
allow one to transcribe a mRNA with a precise 3' end in transfected
cells.  In transfected cells, most of the transcription is dictated by
the poly A signal which results in an addition of a poly A tail.  But I
want to generate a RNA with a precise sequence (i.e. without the
extraneous poly A addition).


Thanks in advance


Anita
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