sphingolipids labeling using CT-B

[Lucien Gendron]

yo.  what you gotta do is uise trhe iodine stain.  try it out!
<rousso at gaiberg.wi.mit.edu> wrote in message
news:877bmi$toi$1 at nnrp1.deja.com...
> Hi,
> I am trying without any success to label the membrane of cells using
> Cholera toxin B-Rhodamine. I tried Hela, 293T and Jurket T-cell.
> I would imagine that the labeling should be straight forward. I am
> incubating the cells with the dye (I did series of dilutions), for 10
> minutes, and then wash with PBS followed by PFA fixation - however, I
> get zero signal.
> I would appreciate any help  - if someone has any experience with this
> reagent.
> Thanks,
> Itay
>
>
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